Abstract
Tissue cultures set up from roots of Rumex alpinus L. were grown on Murashige and Skoog's medium with kinetin and either 2,4-D or NAA
as growth factors. The 2,4-D cultures produced a broader spectrum of hydroxyanthracene,
hydroxynaphthalene and hydroxybenzene derivatives than the NAA cultures. In the 2,4-D
cultures the anthraquinones chrysophanol, physcion and emodin, the dianthrones of
chrysophanol and physcion and their heterodianthrone and the monoglucoside of chrysophanol
were identified. Also the naphthaiene-1,8-diols nepodin, nepodinmonoglucoside and
methoxynepodin have been identified. The occurrence of 2-acetylorcinol and its monoglucoside
was also established. In the NAA cultures accumulation of only chrysophanol, physcion
and 2-acetylorcinol could be proved. The yields of these secondary metabolites produced
in the NAA cultures were determined to be much lower than those produced by the 2,4-D
cultures.
Key Word Index
Rumex alpinus - Polygonaceae - Plant Tissue Cultures - 1,8-Dihydroxyanthraquinones
- Naphthalene-1,8-diols
